Preparation of nematicides formulation from Achillea wilhelmsii for controlling root knot nematodes (Meloidogyne incognita)
A.
SALAHI ARDAKANI
دانشیار بخش تحقیقات گیاهپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی کهگیلویه و بویراحمد، یاسوج، ایران
author
B.
HEIDARI ALIZADEH
Iranian Research Institute of Plant Protection
author
text
article
2018
per
Foliage of Achillea wilhelmsii C. Koch. were dried in the shade and room temperature (about 25°C), powdered and extraction was done in the Soxhlet extractor. Water soluble and non-water soluble components of the extract were separated by Dichloromethane/water. In addition, Tween 85 was added to the extract and EC formulation of 5% was made. Results of the laboratory experiments showed good stability of the formulation. The toxicity of formulation to the root-knot nematode, Meloidogyne incognita, was studied in laboratory and pot experiments. Application rates of formulation were 0.125, 0.25, 0.5, 1, 2, 4, 8 and 16 percent for the laboratory experiment in order to determine their effects on motility and mortality of second-stage juveniles (J2) after 24 and 48 h. In pot experiment, 0.125, 0.25, 0.5, 1, 2 and 4 percent of the formulation were applied on nematode activity and plant growth components of tomato (Solanum lycopersicum). Exposure time was significantly (p<5%) affected J2 mortality. J2 mortality was 49.9% for concentration of 4%. It was 100% for the concentrations of 8% and 16%, after 48 h. In pot experiment, all of the concentrations significantly reduced the nematode parameters and overall population. Treatment of 4% of formulation was toxic to tomato plants in comparison to control. In general, concentrations of the 0.50%, 1% and 2% of the formulation were at par and the bests in controlling root knot nematode and improved tomato growth parameters in the pot condition compare with the control.
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
129
138
https://jaenph.areeo.ac.ir/article_115148_ed592a3568884c421cdebd386ace0403.pdf
dx.doi.org/10.22092/jaep.2018.107437.1118
Comparison of abundance and species diversity of herbivorous arthropods in wheat fields under conservation and conventional tillage systems in Zarghan region (Fars province, Iran)
H.
PEZHMAN
Plant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Shiraz, Iran
author
L.
JOKAR
Seed and Plant Improvement Research Department; Fars Agricultural and Natural Resources Research and Education Center, AREEO, Shiraz, Iran
author
M.
ZARE MOAYEDI
Plant Protection Graduated Student, Faculty of Agriculture, Shiraz University, Shiraz, Iran
author
text
article
2018
per
In this research, the abundance and species diversity of herbivorous arthropods in wheat fields under no tillage (NT), minimum tillage system (MT) and conventional tillage system (CT) were compared during two successive growing seasons. Sampling was done by pitfall traps, yellow sticky cards and D- vac device during wheat growth stages. Diversity indices (Shannon - Wiener, Simpson, and Berger-Parker) and Pileos evenness Index (J) were determined by Past software. Psammotitix aliens (30.1%) was the only eudominant pest species which found in MT system. Dominant pest species in MT were Mallachius bipustulatus (26.13%) and Haplothrips tritici (14%) while in NT were Psammotitix aliens (25.6%), Haplothrips tritici (24.91%), Petrobia latens (12.28%) and in CT were Psammotitix aliens (26.66%) and Mallachius bipustulatus (22.78%)respectively. Shannon index in NT, MT and CT were 2.25, 2.13 and 2.26, While Simpson and Berger- parker indices were 0.84, 0.81, 0.84 and 0.25, 0.30, 0.26 respectively. As well as, J index in mentioned systems were 0.35, 0.31 and 0.34 respectively. In conclusion,’ herbivorous arthropods abundance in CT showed significant difference with MT and NT systems. As well as, diversity and evenness indices in MT showed significant difference with NT and CT systems.
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
139
154
https://jaenph.areeo.ac.ir/article_113579_2e85560b8ec97f2169567184778d98ad.pdf
dx.doi.org/10.22092/jaep.2017.107916.1126
Assessment of antagonistic activity of fluorescent pseudomonads isolated from potato rhizospher towards Ralstonia solanacearum under greenhouse conditions
E.
HASANI
Bou Ali Sina of Hamadan University-Faculty of Agriculture
author
GH.
KHODAKARAMIAN
استاد؛ گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران
author
text
article
2018
per
This study was conducted to evaluate of the antagonistic activity of fluorescent pseudomonads against Ralstonia solanacearum in greenhous. A total of 80 strains of fluorescent pseudomonads were isolated from the soil around the healthy potato roots and tubers collected from 10 farms on King, s B medium (KB). The bacterial R. solanacearum strains were isolated on nutreient agar (NA) medium containing triphenyltetrazolium chloride (TTC). Antagonistic activity of pseudomonads strains was evaluated in a completed randomized design with three replicates in vitro. The bacterial strains EH87 and EH203 with 3.78 and 3. 65 cm growth inhibition zone were the most effective and EH27 and EH40 strain with 1. 85 and 1.54 cm had the least effect. The phenotypic feature of the antagonistic representative’s strains and their hypersensitive reaction on tobacco leaf showed that they were belonged to Pseudomonas putida and P. fluorescens (bv. I, II & IV). Based on the result of laboratory experiment nine representative strains were selected for further studies in a randomized design under greenhouse condition. After three months, growth factors of the plants and disease severity were measured and recorded. Results showed that EH70, EH49 bacterial strains had a high biocontrol effect in compare to contaminated control. They reduced the potato wilt disease caused by R. solanacearum 84% and 75% respectively. Total DNA from antagonistic representaives strains were used as a template to amplify 16SrRNA encoding gene using universal primers in PCR. Results showed that the strain EH53 had 98 percent sequence similarity to Pseudomonas fluoresens.
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
155
166
https://jaenph.areeo.ac.ir/article_113888_0a42bc6c61ca1f5f4cafd384dfb9eb62.pdf
dx.doi.org/10.22092/jaep.2017.108372.1137
Study on the control effect of fennel (Foeniculum vulgaris) and stinging nettle leaf (Urtica dioica) extract against root knot nematode in cucumber plant
N.
GHOBADI
دانش آموختهی کارشناسی ارشد، گروه بیماری شناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران، ایران
author
F.
RAKHSHANDEHROO
استادیار گروه بیماری شناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران، ایران
author
A.
SAEEDI ZADEH
استادیار گروه گیاهپزشکی، دانشکده علوم کشاورزی، دانشگاه شاهد، تهران
author
text
article
2018
per
The nematicidal effect of aquatic and alcoholic extracts of fennel and nettle plants against the root-knot nematode (M. javanica) in cucumber plants was studied under the greenhouse condition. The crude extract of dried leaf material of fennel and nettle crude extracts were extracted by soxhlet apparatus. A number of 1000 second-stage juvenile of the nematode as well as egg masses was added to the hybrid cucumber plant Beit Alpha 192 F1. The aquatic and alcoholic fennel and nettle crude extracts were added directly into the soil rhizosphere in the pods at the concentrations of 100 and 200 ppm, 10 days post inoculation. The chlorophyll content was assessed up to 15 days after extract addition. The second-stage juvenile of the nematode, egg mass, nematode gall and growth factors of the treated plants evaluated two month after extract addition. Experiments were carried out as factorial based on completely randomized design with 3 replications. Results indicated that the disease severity of the nematode reduced three times in comparison to the infected none treated control plant. Significant increase in the growth factors and chlorophyll content in fennel and nettle crude extracts treated plants was also observed. It was also revealed that the fennel crude extracts were more effective in controlling the nematode compared to the nettle extract. Aquatic fennel crude extract at the concentration of 100 ppm showed the best nematicidal activity.
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
167
180
https://jaenph.areeo.ac.ir/article_113692_f172968af8e143a8c0bfccf0b3cfff89.pdf
dx.doi.org/10.22092/jaep.2017.108750.1143
Preparation of nanoemulsion formulation of essential oil of Zataria multiflora and comparison of contact toxicity with pure essential oil on Ephestia kuehniella
L.
EMAMJOMEH
دانش آموخته دکتری گروه حشره شناسی، واحد
علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
author
S.
IMANI
1- استادیار گروه حشره شناسی، واحد
علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران؛
2- گروه انگلشناسی و سمشناسی دانشکده پزشکی، دانشگاه علوم پزشکی، سمنان، ایران
author
KH.
TALEBI
استاد گروه گیاه پزشکی، دانشکده علوم مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران
author
S.
MOHARRAMIPOUR
دانشیار گروه حشره شناسی، دانشکده کشاورزی، دانشگاه تربیت
مدرس، تهران، ایران
author
K.
LARIJANI
استادیار گروه شیمی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
author
text
article
2018
per
In recent decades, providing new formulations of botanical insecticide as a replacement for synthetic pesticides is taken in to consideration. Essential oils have been used as pesticides in last decades, but essential oils have many restrictions on their use such as voltality, rapid oxidation and chemical instability in the prescence of light, moisture and high temperature. In order to increase the efficiency of essential oils, the use of formulations with controlled release of the oil encapsulated in micro- and nano-scales would be the best option.In this research ,after making a new nanoemulsion formulations ofessential oil of Zataria multiflora, contact toxicityof the essential oil before and after nanoemulsion was evaluated on reared Ephestia kuehniella at 27±1°C and 65±5% RH under dark conditions.Nanoemulsion essential oil was carried out by the method of gum - maltodextrin using homogenization and morphology and size were determined by TEM .In contact toxicity test, the LC50 values of pure and nanoemulsion essential oil of Zataria.multiflora on larvae after 72hours ,were 0.61 and 0.15 µl/cm² respectively. Therefore the toxicity of nanoemulsion essential oil of Zataria.multiflora was 4.32 folds higher than the pure essential oil. Findings led to a conclusion that encapsulated thechnology of essential oils can enhance their control release and persistence under controlled condition.
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
181
190
https://jaenph.areeo.ac.ir/article_113550_940f94df366d1ab3d9a5c42a6f8caa7b.pdf
dx.doi.org/10.22092/jaep.2017.109159.1151
Species diversity of parasitoid wasps of Ichneumoniformes (Hymenoptera: Ichneumonidae) in Jiroft county, Kerman province, Iran
M.
MAHYABADI
Department of Plant Protection, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
author
M.
KHAYRANDISH KASHKOOEI
استادیار، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان
author
H. M.
TAKALOOZADEH
Department of Plant Protection, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
author
text
article
2018
per
The present survey, provides species diversity of Ichneumoniformes (Hymenoptera: Ichneumonidae) in some areas of Jiroft (Southeast Iran). Sampling was done during 2014-2015 at different locations and altitudes with Malaise trap. All obtained specimens were identified to species level and also the numbers of each species per sample were counted. The collected specimens were analyzed with the software SDR 4. A total of 20 species from 17 genera are listed in this paper as the fauna of Jiroft. The results of indices of Shannon Winer and Pielou J showed that Maskoon with 17 species and 49 specimens has the highest diversity and evenness, Jiroft with 15 species and 54 specimens, Faryab with 8 species and 13 specimens were the next rates. Among all species Mesostenus grammicus with 31 specimens (22.14%) has the most abundance in all studied area. Also five species of them with only one specimen (0.71%) were determined as subrare species.
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
191
202
https://jaenph.areeo.ac.ir/article_115573_bda61b243b42b1a382a3a1cdeb7b7d35.pdf
dx.doi.org/10.22092/jaep.2018.109631.1156
Pathogenic effects of three Nucleopolyhedrovirus, Spodoptera littoralis NPV, Helicoverpa armigera NPV, Spodoptera litura NPV on life stages of Egyptian cotton leafworm Spodoptera littoralis
Z.
MAGHOLIFARD
Department of Plant Protection, Faculty of Agricultural Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran
author
SH.
HESAMI
Department of Entomology, Faculty of Agricultural Sciences, Shiraz branch, Islamic Azad University
author
R.
MARZBAN
Iranian Research Institute of Plant Protection (IRIPP)
author
GH.
SALEHI JOUZANI
Department of Microbial Biotechnology and Biosaftey , Agricultural Biotechnology Research Institute of Iran (ABRII)
author
text
article
2018
per
In this study, pathogenicity of three virus isolates, Spodoptera littoralis NPV, Helicoverpa armigera NPV, Spodoptera litura NPV was evaluated in three different concentrations of 6× 102, 6× 104, 6×106 OBml-1 on 5-day-old larvae of Egyptian cotton leafworm, S. littoralis at 25 ˚C on artificial diet. The highest larval duration was observed in concentration of 6×104 OBml-1 H. armigera NPV with 19.7 days which had a significant difference with other treatments. S. littoralis NPV in concentrarion of 6×104 had the lowest percentage of pupal rate (10%) and lowest pupal weight in concentrarions of 6×102 and 6×104 OBml-1 (18.3 and 13.8 mg, respectively). The lowest adult emergence (6.7%) was observed in S. littoralis NPV isolate with 6×104 OBml-1 concentration. S. littoralis NPV was most effective virus for controlling various biological stages of this pest. The LC50 of S. littoralis NPV isolate on 5-day-old larvae of Egyptian cotton leafwormwas determined to be 7×102 OBmL-1
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
203
218
https://jaenph.areeo.ac.ir/article_114950_897dd95896558ba84dcda6bc90115de9.pdf
dx.doi.org/10.22092/jaep.2018.109796.1158
The efficacy of some native Trichoderma isolates in induction of resistance in tomato against Fusarium oxysporum f. sp. lycopersici, the causal agent of Fusarium wilt disease
SH
ALEAGHAEE
Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
S.
REZAEE
Department of Plant Pathology
College of Agriculture and Natural Resources
Science and Research Branch
Islamic Azad University
Tehran, Iran
author
M.
EBADI
Department of Biology, College of Basic Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran
author
H. R.
ZAMANIZADEH
Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
text
article
2018
per
Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (Fol) is one of the most destructive tomato diseases. The disease control through induction of resistance by biocontrol agents is a management strategy to reduce application of chemical fungicides. So, in this study the efficacy of 10 native Trichoderma isolates in induction of resistance against the pathogen was evaluated. In greenhouse, phenotypic evaluation of induction of resistance by Trichoderma isolates with stem-injection and split-root bioassay methods showed the least disease severity and incidence significantly (p≤0.05) in Trichoderma harzianum Th-14+Fol and Trichoderma atroviride Ta-30+Fol treatments. The results of study of rhizosphere colonization ability of Trichoderma isolates by serial dilution method, revealed maximum population density in these two isolates. The disease severity showed negative correlation with Trichoderma population density. The activity of polyphenol oxidase and peroxidase increased significantly (p≤0.05) in the plants treated with Th-14 and Ta-30 isolates compared to control (Fol). The results of this study can be applied in integrated management of tomato Fusarium wilt.
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
219
234
https://jaenph.areeo.ac.ir/article_113551_b44dcc59ef44303d7ef8159f52d84f49.pdf
dx.doi.org/10.22092/jaep.2017.110229.1163
Predicting distribution models of two flower flies, Paragus tibialis and Paragus quadrifasciatus (Diptera: Syrphidae) in different climates of Iran
A.
JABBARI
Department of Entomology, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
A. M.
SARAFRAZI
Insect Taxonomy Research Department, Iranian Research Institute of Plant Protection, Tehran, Iran
author
A. A.
SHAMSIPOUR
Faculty of Geography, University of Tehran, Tehran, Iran
author
S.
IMANI
Department of Entomology, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
text
article
2018
per
The genus Paragus with more than 50 known species in Iran has a high potential to control pest and concerve pollinator insects. This study was conducted to fill the information gap of the distribution patterns and habitat preferences of the two species, Paragus quadrifasciatus Meigen, 1822 and Paragus tibialis Fallen, 1817in different climates of Iran. The distribution model was prepared based on species presence records besides seven bioclimatic variables and altitudes. The accuracy of the model was measured using the Area Under Curve Index. The Jackknife test showed the mean temperature of warmest quarter and the precipitation of warmest quarter had the most effect on the distribution patterns of P. quadrifasciatus and P. tibialis respectively. The results showed that semi-arid climates with cold winter and warm summer in northern latitudes of Iran were suitable for the presence of both species, the central and southern regions of Iran with very high temperatures were considered as unsuitable areas for the presence of both species. Area Under Curve indices for both P. quadrifasciatus and P. tibialis were 0.86 and 0.9, respectively, implying the high precision and accuracy of the models for predicting the distribution models of both species in this study.
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
235
250
https://jaenph.areeo.ac.ir/article_113693_b90bebb2ff087f2aca7aecccd26c67ae.pdf
dx.doi.org/10.22092/jaep.2017.115364.1187
Identification of parasitoid wasps of Scale insects (Chalcidoidea ) in fruit orchards of Markazi province
M.
YOUSEFI
Faculty member of plant protection in markazi provice
author
H. A.
VAHEDI
دانشیار گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی کرمانشاه
author
E.
EBRAHIMI
Professor of Research, Iranian Research Institute of Plant Protection
author
R.
SHARIFI
Assistant Professor of Department of Plant Protection, Razi University of Kermanshah
author
text
article
2018
per
The scale insects of the superfamily Coccoidea are among the most important and harmful pests of fruit trees in Iran. In order to collect and identify parasitoid wasps of Coccoidea, a two-year of sampling was carried out during 2015-2016 in Markazi province. The parasitoid wasps were reared from of scale insects and then were collected daily and preserved in 75% alcohol. The results showed that 10 species of parasitoid wasps belonging to 7 genera from 3 families had parasitism activity on Scale insects. Of them, 3 species were reported for the first time from Iran which are marked with asterisk. Identified species of parasitoid wasps are as follows: Encyrtidae: Blastothrix sericea Dalman, 1820; B. truncatipennis (Ferriere, 1955)*; Habrolepis pascuorum Mercet, 1921; Microterys darevskii Trjapitzin, 1968; M. tricoloricornis De Stefani, 1886; Aphelinidae: Aphytis chrysomphali (Mercet, 1912); Coccophagus piceae Erdos, 1956; Pteroptrix dimidiata Westwood, 1833*; P. opaca Erdos, 1956*; Pteromalidae: Pachyneuron muscarum (Linnaeus, 1758).
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
251
264
https://jaenph.areeo.ac.ir/article_115393_b28024962a3fdd2a2a4365fd365cd878.pdf
dx.doi.org/10.22092/jaep.2018.116034.1192
Production of polyclonal phages harbouring antibody fragment genes against Xanthomonas citri subsp. citri using phage display technology
H.
RAEISI
Plant protection,agriculture,guilan university
author
M. R.
SAFARNEJAD
Department of Plant Viruses, Iranian Research Institute of Plant Protection, Agricultural research education and extension organization of Iran. Tehran
author
S. M.
ALAVI
Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran
author
S. A.
ELAHINIA
Department of Plant Protection, College of Agricultural Sciences, Guilan University, Rasht
author
N.
FARROKHI
Departement of Plant Sciences & Biotechnology, Faculty of Life Sciences & Biotechnology, Shahid Beheshti University G.C, Tehran
author
text
article
2018
per
Citrus bacterial canker, caused by Xanthomonas citri subsp. citri (Xcc),is amongst the important diseases of lime orchards in southern parts of Iran. Phage display has been used to produce specific antibodies for detection of pathogen-infected plants as well as development of resistant varieties. An effector, namely pthA and a pilus protein, HrpE, the major critical components of type III secretion (T3S) system with roles in pathogenesis, were chosen as antigens. Recombinant forms of the proteins (pthA and HrpE) were expressed in a bacterial host and purified via affinity chromatography. Tomlinson phage display libraries including single chain variable fragments were used for isolation of the specific antibodies. Biopanning, 3 rounds against pthA and HrpE proteins, allowed enriching antigen-specific phages. The specificity of phages was tested using ELISA. Moreover, the phages were able to detect the plants infected with citrus bacterial canker.
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
265
276
https://jaenph.areeo.ac.ir/article_114001_11d4cc715e1fa507865038d81de5a78f.pdf
dx.doi.org/10.22092/jaep.2017.115980.1194
First report of Campoletis scyticus (Hym.: Ichneumonidae, Campopleginae) from Iran (Short report)
A.
MOHAMMADI-KHORAMABADI
Shiraz University
author
MATTHIAS
RIEDEL
SNSB – Zoologische Staatssammlung München, Münchhausenstr. 21, 81247 München, Germany
author
SH.
HESAMI
Young Researchers and Elite Club, Shiraz Branch, Islamic Azad University, Shiraz, Iran
author
S.
SHAFIEI
Young Researchers and Elite Club, Kerman Branch, Islamic Azad University, Kerman, Iran
author
text
article
2018
per
Campoletis Förster, 1868 is a relatively large genus of the parasitoid wasps of the subfamily Campopleginae (Hym.: Ichneumonidae) containing more than 100 described species worldwide (Riedel, 2017; Yu et al., 2012). Members of this genus parasitize mainly larvae of the families Noctuidae and Geometridae and less common of Pyralidae, Pterophoridae, Zygaenidae and Drepanidae. Some species have been known as the most important biological control agents of crop pests such as Heliothis spp. (Quicke, 2014). The wasps of this genus have a body length of about 5-9 mm with a prominent tooth at apical edge of clypeus in most species (Fig. 1A, B). There have been recorded six species of this genus from Iran (Davatchi & Shojaei, 1968; Masnadi-Yazdinejad et al., 2010; Riedel, 2017). Here, one another species of this genus is newly reported from Kerman province, Iran. Specimens of this species are deposited in the insect collection of Department of Plant Production, College of Agriculture and Natural Resources of Darab, Shiraz University, Iran.
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
277
278
https://jaenph.areeo.ac.ir/article_115394_0105ed02bc56d6233f1e91393ef9179e.pdf
dx.doi.org/10.22092/jaep.2018.120485.1206
Occurrence of wheat tan spot in Moghan plain (Short report)
H.
MOMENI
Scintific memeber of IRIPP
author
M.
RAZAVI
Scientific member of Iranain Research Institute of Plant Protection
author
H.
KAZEMI
Scientific member of IRIPP
author
text
article
2018
per
During field surveys on May 2016, suspected leaf samples of tan spot were collected from infected wheat fields in Bileh-Savar and Germi in Ardabil province and transferred to the laboratory. Suspected wheat leaves were cut into 3-4 cm long pieces, washed under tap water and were placed on wet Whatman filter paper in Petri plates, incubated at room temperature for 24 hours under fluorescent light and then for 24 hours in the dark at 16ºC. Single conidia from the leaf surface were selected and transferred to WA and then V8 / PDA and stored in the dark for 7 days. These plates were kept under nearUV for 24 hours followed by 12 hours at 16 ºC. Dark-olive color ring of fungal conidia formed around the colony with huge amount of conidiophores and conidia. Conidia were yellow, with 5-7 pseudosepta, 14-18 × 100-200 µm and the basal cells were conical. The finding descriptions were in consistence with characteristics explained for P. tritici-repentis. For pathogenicity tests, susceptible cv. Bolani was used in greenhouse conditions. Wheat seedlings were inoculated with conidial suspension of 4×103 conidia/ml at two-leaf stage. Inoculated seedlings covered with black plastic bags for 48 hours and then transferred to the greenhouse and kept for 6 days at 22 ºC with a photoperiod of 16 hours light. P. tritici-repentis isolated again from infected wheat plants. This is the first report of the occurrence of the wheat tan spot with the causal agent of P. tritici-repentis in Moghan plain of Ardabil province
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
279
281
https://jaenph.areeo.ac.ir/article_113552_feef51a02119ff14d289732c9a25c913.pdf
dx.doi.org/10.22092/jaep.2017.107250.1114
Phylogenetic analysis of four Iranian Cucurbit yellow stunting disorder virus isolates based on 3' terminal region of RNA1 (Short communication in English)
T.
KESHAVARZ
Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension
Organization (AREEO), Tehran, Iran
author
K.
BANANEJ
Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension
Organization (AREEO), Tehran, Iran
author
text
article
2018
per
To study phylogenetic analysis of some Iranian isolates of cucurbit yellow stunting disorder virus (CYSDV), 3' terminal region of RNA1 (560 bp in length) of four CYSDV isolates, originated from four different locations of Iran including Boushehr, Kerman (Jiroft), Khuzestan (Dezfool) and Tehran provinces was amplified by RT-PCR using specific pair primers. The 3' terminal region of RNA 1 of four CYSDV was sequenced and compared with other available sequences in GenBank. Nucleotide sequences analysis of CYSDV isolates showed a high nucleotide sequence identity (98- 99%) among all isolates and nucleotide sequences identity among Iranian CYSDV isolates was 99%. Phylogenetic tree constructed by neighbor-joining method using the nucleotide sequence of amplified region indicated that all CYSDV isolates were placed in one group however three of four Iranian isolates including Boushehr, Khuzestan and Tehran were clustered into a distinct subgroup with respect to other isolates.
Applied Entomology and Phytopathology
Iranian Research Institute of Plant Protection (IRIPP)
1026-5007
85
v.
شماره 2
no.
2018
19
24
https://jaenph.areeo.ac.ir/article_115149_deff1f2f8d75296bc4fec923f3f30c2d.pdf
dx.doi.org/10.22092/jaep.2018.110740.1167