شناسایی و بررسی ویژگی‌های فنوتیپی و ژنتیکی چند جدایه‌ی فوزاریوم بیماریزای گیاهی از میزبان‌های مختلف

نوع مقاله: بیماری‌شناسی گیاهی

نویسندگان

1 دانشجوی دکتری بیماری شناسی، گروه بیماری شناسی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 دانشیار گروه گیاهپزشکی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران

3 فارغ التحصیل کارشناسی ارشد بیماری شناسی گیاهی دانشگاه علوم کشاورزی و منابع طبیعی ساری

4 دانشجوی کارشناسی ارشد بیماری شناسی گیاهی دانشگاه علوم کشاورزی و منابع طبیعی ساری

چکیده

به منظور مطالعه‌ی گونه‌های بیماری‌زای جنس Fusarium روی میزبان‌های مختلف نمونه‌های گیاهی آلوده به بیماری‌های قارچی متعددی از استان‌های یزد، کرمان و مازندران طی سال‌های 93-1391 جمع‌آوری شد. هدف از این مطالعه، امکان سنجی گونه‌های بیماری‌زا بر اساس خصوصیات ریخت‌شناسی و مولکولی و انگشت‌نگاری ژنتیکی و فهم ارتباط بین این گونه‌ها بود. نتایج حاصل نشان داد که استفاده از تکنیک rep-PCR و ترادف حاصل از ناحیه‌ی ITS-rDNA روشی مناسب برای شناسایی گونه‌های مختلف فوزاریوم است. همچنین براساس نتایج به‌دست آمده، نمونه‌های مطالعه شده به عنوان هشت گونه‌ تشخیص داده شدند: F. brachygibbosum، F. delphinoides، F. equiseti، F. globosum، F. graminearum، F. incarnatum، F. oxysporum و F. proliferatum. گونه‌های F. brachygibbosum عامل لکه برگی روی خرزهره، F. delphinoides عامل پوسیدگی ریشه روی شبدر زینتی، F. equiseti عامل شانکر روی کرچک ، F. globosum عامل سوختگی غلاف نی، F. incarnatum عامل شانکر تاج خروس، F. oxysporum عامل پوسیدگی برگ روی آلوئه ورا و F. proliferatum به عنوان عامل شانکر روی کنجد، سوختگی برگ روی سیکاس و عامل همراه با پژمردگی آوندی روی نخل برای اولین بار از روی این میزبان‌ها از ایران گزارش می‌شوند. همچنین گونه‌ی F. delphinoides یک گونه‌ی جدید برای فلور قارچی ایران می‌باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of Genotypic and Phenotypic Characteristics of several Plant Pathogenic Fusarium Isolated on Different Hosts

نویسندگان [English]

  • H. A. MIRHOSSEINI 1
  • V. BABAEIZAD 2
  • L. HASHEMI 3
  • E. BASAVAND 4
1 Sari Agricultural Sciences and Natural Resources University
چکیده [English]

In 2011-2013, to study of plant pathogen Fusarium species on different hosts, the samples from Yazd, Kerman and Mazandaran provinces were collected. This study was purposed for identification of plant pathogen Fusarium species on the basis of morphological and molecular characteristics and relationship between of species. The samples were detected to eight different species including: F. brachygibbosum, F. delphinoides, F. equiseti, F. globosum, F. graminearum, F. incarnatum, F. oxysporum and F. proliferatum. F. brachygibbosum as causal agent of oleander leaf spot, F. delphinoides as kidney weed root rot, F. equiseti as stem canker in castor bean, F. globosum as giant cane sheet blight, F. incarnatum as stem canker on redroot pigweed, F. oxysporum as aloe leaf rot and F. proliferatum as causal agents of sesame canker, cycas leaf blight and date palm vascular wilt, for first time have reported in Iran. F. delphinoides is a new species for the mycoflora of Iran.

کلیدواژه‌ها [English]

  • ITS-rDNA
  • Morphological and molecular studies
  • rep-PCR
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