ارزیابی آزمایشگاهی و میدانیBacillus thuringiensis Berliner روی کرم جوانه خوار مرکبات Archips rosanus Linnaeus در استان مازندران

نوع مقاله: حشره شناسی کشاورزی

نویسندگان

1 دانش آموخته کارشناسی ارشد ، گروه گیاهپزشکی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

2 دانشیار؛ گروه گیاهپزشکی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

پروانه جوانه‌خوار Archips rosanus از آفات پلی‌فاژ در حال گسترش در باغ‌های مرکبات غرب استان مازندران می‌باشد. در این تحقیق اثر باکتری Bacillus thuringiensis subsp kurstaki روی مراحل مختلف لاروی کرم جوانه‌خوار مرکبات Archips rosanus مورد بررسی قرار گرفت. برای انجام آزمایش‌های زیست‌سنجی‌ در شرایط آزمایشگاهی از روش غوطه‌ورسازی (Dipping method) استفاده شد.LC50 و LC90 باکتری B.t برای لاروهای سن اول و مجموع سن دوم و سوم لاروی به ترتیب 4480، 9010 و 4460، 19040 پی‌پی‌‌ام به دست آمد. آزمایش گلدانی روی نهال‌های دو ساله رقم پرتقال واشنگتن ناول Citrus sinensis(L) Osbeck.Var novel با 5 تیمار و آب یونیزه به عنوان شاهد و در قالب طرح کاملا تصادفی انجام شد. پس از تجزیه و تحلیل داده‌ها، LC50 و LC90 این باکتری برای لاروهای سن اول و دوم A. rosanus96 ساعت پس از تیمار به ‌ترتیب 2130، 13790 و 2840، 16840 پی‌پی‌‌ام محاسبه شد. آزمایش میدانی در قطعه‌ای 3000 متر مربعی از یک باغ 30 هکتاری انجام شد. در این آزمایش 4 تیمار حشره‌کش مالاتیون (57%=EC) (2 در هزار)، باکتری B. thuringiensis( 5/0 درصد)، (1 درصد) و آب‌پاشی به عنوان شاهد با یکدیگر مقایسه شدند. میانگین تعداد لاروهای زنده A. rosanus روی واحدهای نمونه‌برداری در سال اول اختلاف معنی‌داری نداشته اما در سال دوم در درختان شاهد، B. thuringiensis( 5/0 درصد)، (1 درصد) و مالاتیون 7 روز بعد از تیمار به‌ترتیب a466/5، c4/2، ab4/4 و bc266/3 بودند. نتایج به صورت کلی نشان داد که حشره‌کش میکروبی B.t قابلیت کشندگی مناسبی برای لاروهای آفت مذکور در شرایط باغی دارد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Laboratory and field evaluation of Bacillus thuringiensis Berliner for the control of Archips rosanus Linnaeus in Mazandaran province

نویسندگان [English]

  • R. PIROUZ 1
  • M. R. DAMAVANDIAN 2
  • B. AMIRI BESHELI 2
چکیده [English]

In this study, the effects of,Bacillus thuringiensis Berliner subsp kurstaki on the larval stages of Archips rosanus Linnaeus was investigated. Dipping methodwas usedfor bioassayin vitro. LC50 and LC90 of the first and, the sum of second and third larval instars were shown to be 4480, 9010 and 4460 19040 ppm, respectively. A semi-field experiment on the two-year Washington navel (Citrus sinensis(L) Osbeck.Var novel) plants with 5 treatments and water as control was conducted in a completely randomized design. After data analysis, LC50 and LC90 of the first and the second instars larvae of A. rosanus 96 hours after treatment were 2130, 13790 and 2840, 16840 ppm, respectively. The field experiment was conducted in 3,000 m2 orchard of 30 hectares. In this experiment, four treatments namely, Malathion (2000 ppm), B. thuringiensis (0.5 and 1 percent) and water as acontrol compared with each other. The average number of alive larvae of A. rosanus on sample units trees in the first year had no significant difference, but in the second year on the trees for the control,B. thuringiensis (0.5, 1 percent) and Malathion 7 days after treatment,were 5.466a, 2.4c, 4.4ab and 3.266bc, respectively. Overall results showed that microbial insecticide B.t has killing efficiency for the larvae in the orchards.

کلیدواژه‌ها [English]

  • Bioassay
  • citrus orchard
  • Archips rosanus
  • Bacillus thuringiensis
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