کاربرد توام دوگونه از Trichoderma و Bacillus subtilis در کنترل نماتد ریشه گرهی گوجه فرنگی (Meloidogyne javanica)

نوع مقاله: مدیریت آفات و بیماری‌های گیاهی


1 کارشناس ارشد، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد

2 دانشیار، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد

3 استاد، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد


در تحقیق حاضر، کاربرد همزمان دو گونه Trichoderma harzianum و T. virens به همراه دو جدایه از باکتری Bacillus subtilis، در کاهش شاخص های بیماریزایی نماتد Meloidogyne javanica و بهبود فاکتورهای رشدی گیاه گوجه فرنگی ارزیابی گردید. آزمون سنجش زیستی، کاربرد عصاره کشت گونه های قارچی و سوسپانسیون جدایه های باکتریایی را به صورت تکی و تلفیقی بر تفریخ تخم و مرگ و میر لارو نماتد بررسی نمود. در تیمار ترکیبی T. virens (جدایه T65) و B. subtilis (جدایه MD) مرگ و میر لارو سن دوم و عدم تفریخ تخم نماتد به ترتیب به میزان 5 و 5/1 برابر نسبت به شاهد در شرایط آزمایشگاه افزایش یافت. همچنین ریشه های آلوده گوجه فرنگی رقم ارلی اوربانا در مرحله 4-5 برگی با ترکیب جدایه های ذکر شده، در شرایط گلخانه تیمار گردیدند که نتایج حاکی از کاهش 68، 5/57 و 3/38 درصدی به ترتیب در تعداد گال، تخم و لارو سن دوم نسبت به شاهد دارد. ارزیابی بهبود فاکتورهای رشدی گوجه فرنگی در شرایط گلخانه دلالت بر این دارد که جدایه باکتریایی MD، پتانسیل افزایش 34 و 110 درصدی را به ترتیب در طول ساقه و ریشه گوجه فرنگی در مقایسه با شاهد سالم دارا می باشد. به نظر می رسد جدایه MD باکتری B. subtilis ، گزینه مناسبی جهت بررسی های بیشتر در زمینه تولید هورمون های گیاهی و احتمال القاء مقاومت در گیاه باشد. همچنین برآورد کارایی تیمار تلفیقی T.65+MD در کاهش شاخص های بیماریزایی نماتد ریشه گرهی در شرایط طبیعی پیشنهاد می گردد.



عنوان مقاله [English]

Simultaneous application of two species of Trichoderma and Bacillus subtilis to control root knot nematode (Meloidogyne javanica) on tomato

نویسندگان [English]

  • H. ROUHANI 3
چکیده [English]

In the current research, simultaneous application of Trichoderma harzianum (T.BI) and T. virens (T.65) species with two strains of Bacillus subtilis (MD, Bs) were evaluated on reduction of Meloidogyne javanica pathogenicity indexes and improvement of tomato growth factors in a bioassay trial fungal culture extract and bacterial suspension were tested on mortality of second stage juveniles and egg hatching individually and simultaneously. Application of T65 (T. virens) and MD (B. subtilis) caused 5 and 1.5 times enhancement in juvenile mortality and egg hatching inhibition of M. javanica in comparison to control in laboratory conditions. Furthermore, roots of tomato seedlings with 4-5 leaves were treated by mentioned combination in greenhouse. Comparing to control treatment of T65+ MD lead to reduction of gall, egg and second stage juvenile numbers by 68, 57.5 and 38.3 % respectively. According to our results, efficiency assessment of T.65+MD treatment on reduction of pathogenicity indexes in root knot nematode is recommended under natural condition. Evaluation of tomato growth factors improvement in pot experiments revealed that MD bacterial strain, has potential of increasing tomato shoot and root length by 34 and 110 % respectively in comparison to healthy control. Therefore, it seems that MD strain is suitable candidate for further research in the fields of plant hormones production and possible induction of resistance in plant.

کلیدواژه‌ها [English]

  • Bacillus
  • biological control
  • Trichoderma
  • Root knot nematode
ABBASI, A. A. H. and R. SHARF, 2011. Antagonistic effects of Pseudomonas fluorescens and Bacillus subtilis on Meloidogyne incognita infecting vigna mungo L. International Journal of Plant, Animal and Environmental Sciences. 2: 55 - 63.

ABO-ELYOUSR, K. A., Z. KHAN, M. El-MORSI AWARD and M. F. ABEDEL-MONEIN, 2010. Evaluation of plant extracts and Pseudomonas spp. for control of root-knot nematode, Meloidogyneincognita on tomato. Nematropica, 40:289-299.

ASHOUB, A. H. and M. T. AMARA, 2010. Biocontrol activity of some bacterial genera against root-knot nematode, Meloidogyne incognita. Journal of American Science, 6: 321-328.

BEHNAMIAN, M. and S. MASIHA, 2001. Tomato: with most emphasize on greenhouse tomato. Sotodeh Press.

BOKHARI F. M. 2009. Efficacy of some Trichoderma species in the control of Rotylenchulus reniformis and Meloidogyne javanica. Archives of Phytopathology and Plant Protection, 42: 361-369.

DAMADZADEH, M. 2006. Nematology in agriculture. Andishe gostar, Esfehan. 

El-SHENNAWY, M. Z., E. Z. KHALIFA, M. M. AMMAR, E. M. MOUSA and S. L. HAFEZ, 2012. Biological control of the disease complex on potato caused by root-knot nematode and Fusarium wilt fungus. Nematologia Mediterranea, 40: 169-172.

GARDENER, B. B. M. 2004. Ecology of Bacillus and Paenibacillus spp. in agricultural systems. Phytopathology, 94: 1252–125.

GOLZARI, H., M. PANJEKEH, M. AHMADZAEH, M. SALARI and A. SEDAGHAT, 2011. Study the role of fluorescent Pseudomonas bacteria metabolites root-knot nematode Meloidogyne javanica on tomatoes in control. Iranian Journal of Plant Protection Science, 42(1): 113-124.

GOLZARI, H., M. PANJEKEH, M. AHMADZAEH, M. SALARI and E. SEDAGHTI-KHORAVI, 2011. Elucidating the parasitic capabilities of Trichoderma against Meloidogyne javanica on tomato. Plant Disease, 1: 12-19.

HARMAN, G. E., C. R. HOWELL, A. VITERBO, I. CHET and M. LORITO, 2004. Trichoderma species opportunistic, avirulent plant symbionts. Nature Reviews, 2: 43-56.

HASHEM, M. and K. A. ABO-ELYOUSR, 2011. Management of the root-knot nematode Meloidogyne incognita on tomato with combinations of different biocontrol organisms. Crop Protection, 30: 285-292.

HUANG, Y., C. K. Xu, L. MA, K. Q. Zhang, C. Q. DUAN and M. H. MO, 2010. Characterization of volatiles produced from Bacillus megateriumYFM3.25 and their nematicidal activity against Meloidogyne incognita. European Journal of Plant Pathology, 126: 417–422.

HUSSEY, R. S. and K. R. BARKER, 1973. A comparison of methods of collecting inocula of Meloidogyne spp. including a new technique. Plant Disease Reporter, 57: 1025-1028.

JEPSON, S. B. 1987. Identification of Root-Knot Nematodes (Meloidogyne species). CAB International. United Kingdom. 265p.

JINDAPUNNAPAT, K., B. CHINNASRI and S. KWANKIAE, 2013. Biological control of root-knot nematodes (Meloidogyne  enterolobii) in guava by the Fungus Trichoderma harzianum. Journal of Developments in Sustainable Agriculture, 8: 110-118.

KAPLAN, R. and A. C. THAVARANJIT, 2015. Promotion of vegetable seed germination by soil borne bacteria. Archives Applied Science Research,7:17-20.

LEE, Y. S. and K. Y. KIM, 2016. Antagonistic Potential of Bacillus pumilus L1 against root-knot nematode, Meloidogyne arenaria. Journal of Phytopathology. 164: 29-39.

LIAN, L. H., B. Y. TIAN, R. XIONG, M. Z. ZHU, J. XU and K. Q. ZHANG, 2007. Proteases from Bacillus: a new insight into the mechanism of action for rhizobacterial suppression of nematode populations. Letters in Applied Microbiology, 45: 262-269.

LIEFERT, C., H. LI, S. CHIDBURE, S. HAMSON, S. SIGEE, H. S. EPTON and A. HARBOUR, 1995. Antibiotic production and biocontrol activity by Bacillus subtilis cl. 27 and Bacillus pumilus cl. 45. Journal of Applied Bacteriology, 78: 97-108.

MAJZOOB, S. H., A. KARGAR BIDEH, H. A. ZARGHANI, 2010. Effect of Pseudomonas fluorescens CHAO, Bacillus subtilis and Pantoea sp. in the control of Meloidogyne javanica on cucumber (Super Amelia and Royal). In: 19th Iranian Plant Protection Congress, Tehran, Iran 533. (In Persian with English summary).

MEYER, S. L. and D. P. ROBERTS, 2002. Combinations of biocontrol agents for management of plant-parasitic nematodes and soilborne plant-pathogenic fungi. Journal of Nematology, 34:1–8.

MIRAKI, K., M. ABDOLLAHI and F. TALLAYI, 2013. Effect of Trichoderma virens and Glomus mosseae in controlling root knot nematode (Meloidogyne javanica) in tomato. Biological Control of Pests and Plant Diseases, 2: 9-16.

MOKHTARI, S., N. SAHEBANI and H. ETEBARIAN, 2014. Biological control of Meloidogyne javanica by two against antagonists Trichoderma harzianum BI and Pseudomonas fluorescens CHAO in tomato plant. Biological Control of Pests and Plant Diseases, 3: 117-126.

MUNSHID, H., S. SIMON and A. A. LAL, 2013. Antagonistic potential of Bacillus subtilis and Pseudomonas fluorescens on Meloidogyne incognita of green onion (Allium fistulosum). International Journal of Botany and Research, 3: 15-22.

MUTHULAKSHMI, M., K. DEVRAYAR and E. I. JONATHAN, 2010. Biocontrol of root-knot nematode, Meloidogyne incognita (Kofeid and White) Chitarod in mulberry. Journal of Bio pesticide, 3: 479-482.

NASERINASAB, F., N. SAHEBANI and H. R. ETEBARIAN, 2011. Biological control of Meloidogyne javanica by Trichoderma harzianum BI and salicylic acid on Tomato. African Journal of Food Science, 5: 276 – 280.

NIU, Q., X. HUANG, L. ZHANG, L. LIAN, Y. LI, J. LI and K. ZHANG, 2007. Functional identification of the genebace16 from nematophagous bacterium Bacillus nematocida. Applied Microbiology and Biotechnology, 75: 141–148.

RADWAN, M. A., S. A. A. FARRAG, M. M. ABU-ELAMAYEM and N. S. AHMED, 2012. Biological control of the root-knot nematode, Meloidogyne incognita on tomato using bioproducts of microbial origin. Applied Soil Ecology 56: 58– 62.

RAMEZANI-MOGHADDAM, M., E. MAHDKHANI-MOGHADAM, S. BAGHAEE-RAVARI and H. ROUHANI, 2013. The nematicidal potential of local Bacillus species against the root-knot nematode infecting greenhouse tomatoes. Biocontrol Science and Technology, 24: 279-290.

RUIZ, S. E., A. J. CRISTOBAL, R. A. REYES, S. J. TUN, R. A. GARCIA and A. J. PACHECO, 2014. In vitroantagonistic activity of Bacillus subtilis strains isolated from soils of the Yucatan Peninsula against Macrophomina phaseolina and Meloidogyne incognita. Phyton, 83:45-47.

SAHEBANI, N. and N. HADAVI, 2008. Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Soil Biology and Biochemistry, 40: 2016-2020.

SHARON, E., M. BAR-EYAL, I. CHET, A. HERRERA-ESTRELLA, O. KLEIFELD and Y. SPIEGEL, 2001. Biocontrol of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Phytopathology, 91: 687-693.

SIKORA, R. A. and E. FERNANDEZ, 2005. Nematode parasites of vegetables. In: Plant parasitic nematodes in subtropical and tropical agriculture. Luc, M., Sikora. R. A. and Bridge, J. (Editors), 2nd edition, CABI Wallingford, UK.

SINGH, P. and Z. A. SIDDIQUI. 2010. Biocontrol of root-knot nematode Meloidogyne incognita by the isolates of Bacillus on tomato. Archives of Phytopathology and Plant Protection, 43: 552-561.

TYLOR, A. L. and J. N. SASSER, 1978. Biology, identification and control of root-knot nematode, Meloidogyne species. North Carolina State University Graphics, Raleigh, NC. 111pp.

WHITEHEAD, A. G. and J. R. HEMMING, 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology, 55: 25-38.