تاثیر ویروس مخطط توتون بر غلافبندی ارقام مختلف سویا در شرایط گلخانه

نوع مقاله: بیماری‌شناسی گیاهی

نویسندگان

1 دانشجوی دکتری- گروه گیاهپزشکی، دانشکده علوم کشاورزی وصنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه گیاهپزشکی، دانشکده علوم کشاورزی وصنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

3 بخش تحقیقات ویروس شناسی، موسسه تحقیقات گیاهپزشکی، سازمان تحقیقات، آموزش و ترویج کشاورزی

4 گروه بیوتکنولوژی و اصلاح نباتات، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

چکیده

ویروس مخطط توتون (Tobacco streak virus) یکی از مهم‌ترین ویروس‌های مخرب محصولات زراعی بوده و به‌عنوان عامل سوختگی جوانه‌های سویا در نقاط مختلف دنیا گزارش شده است. در این پژوهش، واکنش ارقام رایج سویا در شمال کشور نسبت به این ویروس و تأثیر احتمالی آن بر سویا در شرایط گلخانه ارزیابی شد. پژوهش به‌صورت طرح کاملاً تصادفی در قالب فاکتوریل با دو تیمار جدایه‌های ویروس (جدایه‌های سویا، ماش و آفتابگردان) و ارقام سویا (ویلیامز، امیر، کتول، سامان، ساری) در سه تکرار انجام شد. برای اثبات آلودگی بوته‌ها از آزمون الایزای مستقیم با استفاده از آنتی‌سرم چند همسانه‌ای TSV و واکنش زنجیره‌ای پلیمراز با استفاده از آغازگرهای اختصاصی ویروس استفاده شد. نتایج نشان داد جدایه‌های ویروس به‌طور معنی‌داری باعث کاهش ارتفاع بوته‌ها، کاهش تعداد گره‌ها  و کاهش تعداد غلاف‌های سالم شدند. ارقام نیز دارای تأثیرمعنی‌دار برشاخص‌های رشدی ارتفاع بوته ، تعداد گره، تعداد غلاف‌سالم، و وزن تر بوته‌ها بودند. کمترین میزان تفاوت شاخص‌های رشدی و میزان غلاف‌بندی نسبت به نمونه شاهد در رقم ویلیامز و بیشترین آنها در ارقام امیر و کتول مشاهده گردید. شدت علایم ایجاد شده توسط جدایه سویا بیشتر از دو جدایه ماش و آفتابگردان بود. نتایج حاکی از تأثیر معنی‌دار جدایه‌های ویروس و ارقام بر غلاف‌بندی سویا بود.
 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Tobacco streak virus on in different soybean cultivars in greenhouse conditions

نویسندگان [English]

  • Samira Shameli 1
  • Farshad Rakhshandehroo 2
  • Mohammadreza Safarnejad 3
  • Seyedeh Sanaz Ramezanpoor 4
1 Department of Plant Protection, College of Agriculture Sciences and Food Industries , Science and Research Branch, Islamic Azad university, Tehran, Iran
2 Department of Plant Protection, College of Agriculture Sciences and Food Industries , Science and Research Branch, Islamic Azad university, Tehran, Iran
3 Department of Plant virology, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
4 Faculty of plant production,Gorgan University of Agricultrul Sceince and Natural Resources, Gogan, Iran
چکیده [English]

Tobacco streak virus is one of the most important destructive viruse of crops, and reported as a causal agent of soybean bud blight in different region of world. In this study, the response of soybean cultivars, that were common in the north of Iran, was evaluated to TSV and the possible effect of virus on soybean, under greenhouse conditions. This research was carried out in two way factorial arrangement on a CRD (completely randomized design) with virus isolates (soybean, mungbean and sunflower isolates) and soybean cultivars (Williams, Amir, Katol, Saman, Sari) as treatments in 3 replications. Plants were tested for TSV infection by DAS-ELISA method with specific polyclonal antibody, and polymerase chain reaction (PCR) with specific designed primer. Results showed that viral isolates significantly reduced the plant length, number of nodes, and number of healthy pods. Cultivars had significant effect on growth indices: plant length, number of nodes, number of pod and fresh weight, too. The lowest differences in growth indices and podding in compare of control group observed in Williams cultivar whereas the highest seen in Amir and Katol cultivars. The symptoms caused by soybean isolate were more severe than mungbean and sunflower isolates. Results indicated significant effect of viral isolates and soybean cultivars on podding.

کلیدواژه‌ها [English]

  • Greenhouse
  • podding
  • soybean
  • Tobacco streak virus (TSV)

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