برهمکنش زیر گروه های I و II ویروس موزاییک خیار (CMV) در Nicotiana rustica

نوع مقاله: بیماری‌شناسی گیاهی

نویسندگان

1 گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

2 عضو هیات علمی گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران

چکیده

در این تحقیق بر همکنش دو جدایه Zin1 و Zin2، به‌ترتیب متعلق به زیر گروه‌های I و II ویروس موزائیک خیار (CMV)، روی بوته‌های Nicotiana rustica مطالعه شد. پس از مایه­زنی مکانیکی انفرادی و دوگانه جدایه‌های خالص ویروس روی برگ‌های توتون، نرخ آلودگی و غلظت نسبی هر جدایه به روش آزمون الایزای کمی در شش بازه زمانی مختلف پس از مایه زنی در تیمارهای مختلف بررسی شد. تحلیل آماری داده­های تکرار شونده نشان داد که متغیرهای بازه زمانی و تیمار روی فراوانی ردیابی و غلظت نسبی هر دو جدایه تأثیر معنی­داری (05/0p<) داشت. در تیمارهای دوگانه مایه زنی، نسبت غلظت هر جدایه در گیاهان دوگانه آلوده به تک آلوده (D/S) کمتر از یک بود. در مقاطع زمانی 5، 10 و 18 روز پس از مایه­زنی (dpi)، غلظت نسبی هر جدایه در تیمار تک مایه­زنی با اختلاف معنی­داری (01/0p<) بیش از تیمارهای دوگانه مایه­زنی بود. نتایج آزمون وسترن بلات نیز حاکی از افزایش غلظت ویروس در dpi 18 بود. این نتایج بیانگر برهمکنش دگرپادی بین جدایه های دو زیرگروه این ویروس می‌باشد. نتایج تجزیه واریانس داده‌ها نشان داد که برخلاف شاخص های ناحیه زیر منحنی پیشرفت بیماری (AUDPC) و غلظت کلروفیل، شاخص های شدت بیماری (DSI)، ارتفاع و وزن تر بوته­ها بین تیمارهای مختلف اختلاف معنی­داری نشان دادند. در آزمون های انتقال جدایه ها با شته جالیز از منابع تک و دوگانه آلوده هر جدایه، اختلاف معنی­داری بین نرخ انتقال (T) و کارایی انتقال تک شته (P*) هر جدایه مشاهده نشد.
 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Interaction between Cucumber mosaic virus subgroup I and II in Nicotiana rustica

نویسندگان [English]

  • Susan Tavakoli 1
  • Akbar Dizadji 2
  • Gholam Hossein Mossahebi 1
  • Niayesh shahmohammadi 1
1 Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Department of Plant Protection, college of Agriculture and Natural Resources, University of Tehran
چکیده [English]

Interaction among two isolates of Cucumber mosaic virus (CMV), Zin1 and Zin2 belong to CMV subgroup I and II was investigated on Nicotiana rustica plants in current study. Following singly and doubly mechanical inoculation of pure isolates on N. rustica plants, infection rate (frequency of detection) and relative concentration of each CMV isolate were calculated in six different time trials (days post inoculation, dpi) by quantitative ELISA. Statistical analysis of repeated measures data showed a meaningful effect (p<0.05) of treatment and time variants on frequency of detection and relative concentration of each isolates. D/S ratio for each CMV isolate was less than 1 in doubly inoculation treatment. The results showed that the relative concentration of each isolate in singly inoculated plants was significantly (p<0.01) more than doubly inoculated ones at 5, 10 and 18 dpi. The increase in virus titre at 18 dpi was confirmed by western blot analysis. These results demonestrated a cross-protection interaction among CMV subgroups. Variance analysis of data showed that unlike area under the disease progress curve (AUDPC) and concentration of chlorophyll, disease severity index (DSI), height and fresh weight of plants showed a significant difference among different tratments. In aphid transmission assays by Aphis gossypii fed on singly and doubly infected source plants, no significant difference was observed among transmission rate (T) and single aphid transmission efficiency (P*) of each CMV isolates.
 

کلیدواژه‌ها [English]

  • Keywords: Aphid transmission
  • CMV subgroup
  • Co-infection
  • Disease severity index
  • relative concentration

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