عنوان مقاله [English]
Common bean root rot which is a fungal disease is an economically important diseases of the crop product worldwide. Distribution and relative dominance of the disease was investigated in the farms of the major bean cultivating regions and clima of Lorestan province including Azna, Aligudarz, Selseleh, Doroud and Borujerd. Totally, 1164 isolates were obtained using general and selective culture. Pathogenicity of isolates evaluated and confirmed on Akhtar cultivar. The dominant pathogenic fungi were identified based on colony morphology and microscopic characteristics, and then confirmed by molecular technique using β-tubulin and TEF1- αgenes. Frequency of fungal isolate and the pathogenicity tests showed that F. solani, with 33.42 % isolation frequency was the most important causal agent of common bean root and crown rot in Lorestan province, and followed by F. oxysporum, Macrophomina phaseolina and Rhizoctonia solani with 26.55, 15.55 and 7.73 %, respectively. Frequency of roor rot pathogens distributed across bean farms of Selseleh, Borujerd, Azna, , Doroud and Aligudarz were 24.57, 18.64, 16.58, 15.20 and 8.25 %. respectively. The frequency of the pathogen isolated from common bean plants at the early growth, flowering and podding stages was 20.70, 26.37 and 52.92 %, respectively. Based on Climate zoning, the highest frequency of fungal agents was evaluated in semi-humid temperate clima.
AHMADI, K., GHOLIZADEH, H., EBADZADEH, H., HOSEINPOOR, R., ABDESHAH, H., KAZEMIAN, A. and RAFIEE, M. 2016. Agriculture statistics. Ministry of Jihad e Agriculture of Iran. Tehran, Iran.Vol:1, 125p.
ALIZADEH, A., KAMALI, GH., MOUSAVI, F. and MOUSAVI, M. 2012.Climatology. Publication of Ferdowsi Mashhad University, 392p.
AMANIFAR, N., GHADIRIAN, M. And SALEHI, F. 2017. Commonbean cultivars and genotypes reaction to Fusarium solani f.sp. phaseolina with and without wet stress in greenhouse condition. Plant Pests and Diseases, 85(1):56-68.
ARIF, M., CHAWLA, S., ZAIDI, N.W., RAYAR, J.K., VARIAR, M. and SINGH, U.S. 2012. Development of specific primers for genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1α) gene. African Journal of Biotechnology Vol. 11(2), pp. 444-447.
BEAS-FERNANDEZ, R., DE SANTIAGO-DE SANTIAGO, A., HERNANDEZ-DELGADO, s. and MAYEK-PEREZ, N. 2006. Characterization of Mexican and non-mexican isolates of Macrophomina phaseolina based on morphological characteristic, pathogenicity on bean seeds and endoglucanase genes. Journal of Plant Pathology. 88(1): 53-60
BILGI, V.N., BRADLEY, C.A., KHOT, S. D., GRAFTON, K.F. and RASMUSSEN, J.B. 2008. Response of dry bean genotypes to Fusarium root rot, caused by Fusarium solani f,sp. phaseoli, under field and controlled conditions. Plant Disease. 92: 1197-1200.
BRIEN, R.G.D., HARE, P.J.O., GLASS, R.J. 1991. Cultural practices in the control of bean root rot. Australian Journal of Experimental Agriculture. 31: 551-555.
BURGESS, L.W., SUMMERELL, B.A., BULLOCK, S., GOTT, K.P. and BACKHOUSE, D. 1994. Laboratory Manual for Fusarium Research. Fusarium Research Laboratory. Department of Crop Science, University of Sydney and Royal Botanic Gardens, 133 p.
CAMPBELL, C. L. and NEHER, D. A. 1994.Estimating disease severity and incidence. In: Epidemiology and management of root diseases, edited by Campbell C. L. and Benson D. M. Springer Verlag Berline. Heidelberg, New York, 344pp.
DEACON, J.D. 1997. Modern Mycology. Blackwell science Ltd, Australia 303p.
DAS, I. K., FAKHRODDIN, B., and ARORA, D.K.2008. RAPD Cluster analysis and chlorate sensitivity of some indian isolates of Macrophomina phaseolina from sorghum and their relationships with pathogenesity. Microbiologica Research, 163(2): 215-224.
DEHGHANI, A. 2011. Importance of predisposition in plant diseases incidence (Khuzestan case study).proceeding of 20th IranianPlant Protection Conference, Shiraz, Iran.
DHINGRA, O. D. and SINCLAIR, J.B. 1995. Basic Plant Pathology Methods. CRC Lewis Publishers. 433p.
DHINGRA, O. D., COELHO-NETTO, R. A., RODRIGUES, F. A. SILVA JR, G. J. and MAYA, C. B. 2006. Selection of endemic nonpathogenic endophytic Fusarium oxysporum from bean roots and rhizosphaer competent fluorescent Pseudomonas species to suppress Fusarium-yellow of beans.Biological Control, 39:75-86.
DIX N. J. and WEBESTER. J. 1995. Fungal ecology. Chapman and Hall, 549p.
DOMSCH, K.H., GAMS, W. and ANDERSON, T.H. 1980. Compendium of Soil Fungi. Verlag Eching Germany. 672p.
DOYLE, J.J. and DOYLE, J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15.
EDEL-HERMANN, V., GAUTHERON, N., MOUNIER, A. and STEINBERG, C. 2015. Fusarium diversity in soil using a specific molecular approach and a cultural approach. Journal of Microbiological Methods. 11: 64-71.
ERPER, I., KARACA, G.H. and OZKOC, I. 2008. Root rot disease incidence and severity on some legume specks grown in samsun province and the fungi isolated from roots and soils. Archives of Phytopathology and Plant Protection. 41(7): 501-506
ERSHAD, J. 2010. Fungi of Iran. Agricultural research, education and extention publication. Ministry of Jihad e Agriculture of Iran. Tehran, Iran. 874p.
ESTEVES DE JENSEN, C., KURLE, J.E. and PERCICH, J.A. 2004. Integrated management of edaphic and biotic factors limiting yield of irrigated soybean and dry bean in Minnesota. Field Crop Research. 86: 211-224.
FOOD AND AGRICULTURE ORGANIZATION (FAO). 2015. FAO statistics. Available in: http//www.fao.org
GLOSS, N.L. and DONALDSON, G.C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied Environmental Microbiology. 64(4):1323-1330.
GULER-GUNEY, I. and GULDUR, M.E. 2018. Inoculation techniques for assessing pathogenicity of Rhizoctonia solani, Macrophomina phaseolina, Fusarium oxysporum and F. solani on peper seedlings. Turkish Journal of Agricultural Research 5(1): 1-8.
HALL, R. 1996. Inoculum dynamics of Fusarium solani f.sp. phaseoli and management of Fusarium root rot. In: Hall,R.(Ed.), Principle and Practice of Managing Soilborn Plant Pathogens.The American Phytopathological Society, St. Paul, MN, pp. 279- 320.
HALL, R. and PHILLIS, L.G. 1992. Effect of crop sequence and rainfall on population dynomics of Fusarium solani f.sp. phaseoli in soil. Canadian Journal of Botany.70: 2005-2008.
HOLLIDAY, P. and PUNITHALINGAM, E. 1970. Macrophomina phaseolina. Descriptions of pathogenic fungi and bacteria: CMI 275:1-2.
HARVESON, R.M., SMITH, J.A. and STROUP, W.W. 2005. Improving root health and yield of dry bean in the Nebraska panhandle with a new technique for reducing soil compaction. Plant Disease. 89: 279-284.
KARI DOLATABAD, H., JAVAN-NIKKHAH, M. and SHIER, W. T. 2017. Evaluation of antifungal, phosphate solubilisation, and siderophore and chitinase release activities of endophytic fungi from Pistacia vera .Mycology Progress, published online: 21 June 2017.
KHODAGHOLI, M., HEMMATI, R., NASERI, B. and MAREFAT, A. 2013. Genotypic, phenotypic and pathogenecity variation of Fusarium solani isolates, the causal agent of bean root rot in Zanjan province. Iranian Journal of Pulses Research, 4(1): 111-125.
LAK, M.R. 2003. Common bean root rot diseases.Markazi Agricultural and Natural Resources Research and Education Center.Extention Publication.20p.
LAK, M.R. 2016. Determination of the main agents of bean root rot and evaluation of potential and damage in khomein. Final report of project. Markazi Agricultural and Natural Resources Research and Education Center.20p.
LESLIE, J.F., SUMMEREL, B.A. 2006. The Fusarium laboratory manual. Blackwell Publishing Ltd.: USA. 388p.
MACEDO, R., PATRICIA SALES, L., YOSHIDA, F. LEMES SILVA-ABUD, L. AND JUNIOR, M. L.2017. Potential worldwide distruction of Fusarium dry root rot in common beans based on the optimal environment fir disease occurrence. Available in: http://journals.plos.org/plosone/article?id=10.137/journal.pone.0187770
MC DONALD, J. D. 1994. The soil environment. In: Epidemiology and management of root diseases, edited by Campbell C. L. and Benson D. M. Springer Verlag Berline. Heidelberg, New York, 344pp.
MILA, A.L., CARRIQUIRY, A.L. and YANG, X.B. 2004. Logistic regression modeling of prevalence of soybean Sclerotinia stem rot in the northcentral region of the United States. Phytopathology. 94, 102-110.
MOHAJER SHOAAEI, M. H., REZAEE, A., RAHIMI, A. M., EMAMYARI, K. and SALEHI, P. 1992. Evaluation of resources and land ability of Lorestan regions. Publication no. 608, 75p.
MWANG OMBE, A.W., THIONG, G., OLUBAYO, F.M. and KIPROP, E.K. 2007. Occurrence of root rot disease on common bean(Phaseolus vulgaris L.) in association with bean stem maggot (Ophyiomia sp.) in EMBUdistrict, Kenya. Plant Pathology Journal. 6:141-146.
NASERI, B. 2008. Root rot of common bean in Zanjan, Iran: major pathogens and yield loss estimates. Australian Plant Pathology. 37: 548-551.
NASERI, B. 2013. Epidemics of rhizoctonia root rot in association with biological and physicochaemical properties of field soil in bean crops. Journal of Phytopathology, 161:397-404.
NASERI, B. and MOUSAVI, S.S. 2015. Root rot pathogens in ﬁeld soil, roots and seeds in relation to common bean (Phaseolus vulgaris), disease and seed production. International Journal of Pest Management. Published online:http://www.tandfonline.com
NELSON, P.E., TOUSOUN, T.A. and MARASAS, W.F.O. 1983. Fusarium species, An Illustrated Manual for Identification. Pennsylvania State University Press, University Park.193 p.
ODNNELL, K., KISTLER, H.C., CIGELINK, E. and PLOETS, R.C. 1988. Multiple evolutionary origins of the fungus causing Panama disease of banana: cocordantevidence from nuclear and mitochondorial gene genealogies. Proc. Natl. Acad. Sci. 95(5):2044-2049.
O’DONNELL, K. 2000. Molecular phylogeny of the Nectria haematococcaFusarium solani species complex. Mycologia 92:919–938.
O’DONNELL, K., TODD, J. W., VINCENT, A. R., ROBERT, G., PEDRO, W., CROUS, A., DAVID, M. G. and KANG, S. 2015. DNA sequence-based identification of Fusarium: Current status and future directions. Phytoparasitica, 43:583–595
PAN, R.S. and MORE, T.A. 1996. Screening of melon (Cucumis melo L.) germplasm for multiple disease resistance. Euphytica. 88(2):125-128.
PAPARU, P., ACUR, A., KATO, F., ACAM, C., NAKIBUULE, J., MUSOKE, S., NKALUBO, S. and MUKANKUSI, C. 2017. Prevalence and incidence of four common bean root rots in Uganda. Expelenation of Agriculture. Avalable in: https://creativecommons.org
REZAEE, A. M. 1995. Concepts of probability and statistics. Mashhad publishing co. 431p.
SABBAGHPOUR, S. H. 2014. National document of iranian research strategy. Publication of Agricultureal Research, Education and Extention Organization(AREEO), 417p.
SAFARLOO, Z. and HEMMATI, R. 2014. Identification and pathogenesis study of Fusarium species related to commonbean root rot in Zanjan Province,Aplied Research in Plant Protection, 3(1): 77-92.
SALAGEGHEH, F., MOHAMMADI, H. and SARCHEMEHPOUR, M. 2014. Effect of water stress on root rot disease of Pistacio seedling caused by Fusarium solani. Iranian Journal of Plant Pathology, vol. 50(3):266-280.
SAREMI, H., AMIRI, M. E. and ASHRAFI, J. 2011. Epidemiological aspects of bean decline disease caused by Fusarium species and evaluation of the bean resistant cultivars to disease in Northwest Iran. African Journal of Biotechnology Vol. 10(66), pp. 14954-14961.
SAREMI, H. and SAREMI, H. 2013. Isolation of the most common Fusarium species and the effect of soil solarization on main pathogenic species in different climatic zones of Iran. Europian Journal Plant Pathology. 137: 585-596.
SCHWARTZ, H.F., STEADMAN, J. R., HALL, R. and FORSTER, R.L. 2005. Compendium of Bean Diseases. APS Press, St. Paul, Minnesota, USA. 109 p.
SHEKARI, F., ESMAEELPOUR, B. and SHEKARI, F. 2010. The physiology of vegetable crops. Vol 2.Danesh Publication, Zanjan. 336p.
SINGELTONE, L.L., MIHAIL, J.D. and RUSH, Gh. M. 1993. Methods for research on soil born phytopathogenic fungi. APS Press. St Paul. Minnessota, 263p.
VAN SCHOOVEN, A. and PASTOR-CORRALES, M.A. 1987.Standard system for the evaluation of Bean Germplasm. Cali, Colombia, Centro International de Agricultura Tropical.
VINCELLI, P. and TISSERAT, N. 2008. Nucleic acid-based pathogen detection in applied plant pathology. Plant Disease, 92(5): 660-669.
WERNER, M. and IRZYKOWSKA, L. 2007. The pathogenecity and DNA polymorphism of Fusarium oxysporum originating from Dianthus caryophyllus, Gypsophila spp. and soil. Phytopathol. Pol. 46: 25–36
ZAND, 2016. Identification of homogynous climatic zones in Lorestan Province. Final Report, Lorestan Agricultural and Natural Resources and Education Center. 20p.
ZHANG, N., O’DONNELL, K., SUTTON, D. A., NALIM, F. A., SUMMERBELL, R. C., PADHYE, A. A. and GEISER, D. M. 2006. Members of the Fusarium solani Species Complex That Cause Infections in Both Humans and Plants Are Common in the Environment. Journal of Clinical Microbiology, 2186–2190.