تعیین ویژگی‌های Pseudomonas syringae pv. porri عامل لکه برگی و سوختگی باکتریایی پیاز دراستان‌های فارس و کرمانشاه

نوع مقاله: بیماری‌شناسی گیاهی

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

2 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

چکیده

علائم لکه برگی و سوختگی باکتریایی روی بوته‌های پیاز Allium cepa L.، در استان­های فارس (اقلید) و کرمانشاه (بیستون) با نرخ وقوع 90 تا 100 درصد روی بوته­های پیاز مشاهده شد. به‌منظور جداسازی و شناسایی عامل بیماری، نمونه­های مشکوک از سطح مزرعه­های آلوده جمع‌آوری گردید. پس از کشت روی محیط آگار غذایی، 30 جدایه باکتریایی جداسازی شد. آزمون بیماری‌زایی جدایه­ها روی تعدادی از گونه‌های Allium spp. انجام و بیماری­زایی آن­ها روی پیاز، پیازچه و تره­فرنگی اثبات گردید. این جدایه‌ها از نظر الگوی پروتئین سلولی الکتروفورز شده به روش SDS-PAGE یکسان بودند. براساس ویژگی‌های فنوتیپی مانند تولید رنگدانه فلورسنت روی محیط King’B، آزمون LOPAT و استفاده از منابع کربنی مختلف، جدایه­ها به Pseudomonas syringae pv. porri شبیه بودند. همه جدایه­ها با استفاده از BOX-PCR الگوی باندی یکسانی را نشان دادند. نتایج حاصل از تکثیر و توالی­یابی ژن کد کننده 16S rRNA و ژن­های خانه­داری gyrB و rpoD نشان داد که جدایه­های مورد بررسی متعلق به P. syringae pv. porri هستند. این اولین گزارش از وجود باکتری P.  syringae pv. porri در استان کرمانشاه می­باشد. مدیریت بیماری برای جلوگیری از اپیدمی آن ضروری به‌نظر می‌رسد.
 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Characterization of Pseudomonas syringae pv. porri as the causal agent of bacterial leaf spot and blight of onion in Fars and Kermanshah provinces of Iran

نویسندگان [English]

  • Yousef Moradi Amirabad 1
  • Gholam HKhodakaramian 2
1 Department of Plant Protection, College of Agriculture, Bu-Ali Sina University, Hamadan, Iran
2 Department of Plant Protection, College of Agriculture, Bu-Ali Sina University, Hamadan, Iran
چکیده [English]

The symptoms of bacterial leaf spot and blight on onion (Allium cepa L.) plants were observed in Fars (Eghlid) and Kermanshah (Bistoun) provinces with the rate of occurrence varying from 90 to 100% of the plants. In order to isolation and identification of the causal agent, suspicious samples were collected from infected fields. By culturing the samples on nutrient agar medium, 30 bacterial isolates were obtained. Pathogenicity test of the isolates on Allium spp. was performed and their pathogenicity was proved on onion, spring onion and leek.  The isolates showed an identical total cell soluble protein pattern in SDS-PAGE. The isolates were similar to Pseudomonas syringae pv. porri based on phenotypic characteristics such as production of fluorescent pigment in KB medium, LOPAT tests, and utilization of different carbon sources. Genomic profiles generated with BOX-PCR placed the isolates into one groups. The results of the amplification and sequencing of the 16S rRNA, gyrB, and rpoD genes showed that the isolates belong to P. syringae pv. porri. This is the first report of the presence of the P. syringae pv. porri in Kermanshah province. Management of the disease seems to be necessary to prevent the disease epidemy.
 

کلیدواژه‌ها [English]

  • Allium cepa
  • blight
  • Pseudomonas syringae pv. porri
  • LOPAT
  • phylogenetic

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