عنوان مقاله [English]
Rice sheath blight disease caused by Rhizoctonia solani AG-1 IA has been recognized as a major damaging factor in many rice regions of the world. The aim of this research was investigatingon the effect of two isolates of Pseudomonas protegenes, antagonistic and inducer isolates alone and in combination with potassium silicate on expression rate and production of chitinase, β- 1, 3- gluconase and peroxidase enzymes at 0, 6, 12, 24, 48 and 72 hours after inoculation of Fajr cultivar with the causal agent of rice sheath blight disease.The amount of 1000 and 200 mg of sheath- leaf tissue was prepared as powdered in liquid nitrogen and thenin sodium acetate and phosphate buffers respectively to measure chitinase, β- 1, 3- gluconase and peroxidas eenzymes and 100 mg of the powder in RNAX-Plus solution to study the genes expression and RNA extraction. The amount of β- 1, 3- gluconas eenzyme in 72 hours and chitinase enzyme in 48 hours of infection was at maximum level. As regards, compared with other treatments, the highest level of expression of the genes for chitinase and peroxidase was observed in treatment of antagonistic bacteria in combination with potassium silicate in the early hours of the pathological process, 6 hours after inoculation with the fungal agent, and β- 1, 3- gluconase reached the maximum at 24 h, Thus this treatment recognized as the best treatment.
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