تعیین ویژگی های مولکولی و پراکنش جغرافیایی ویروس کوتولگی سبزرد نخود در ایران

نوع مقاله: بیماری‌شناسی گیاهی

نویسندگان

1 بخش تحقیقات ویروس‌های گیاهی، موسسه تحقیقات گیاهپزشکی کشور، تهران

2 گروه گیاهپزشکی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

3 عضو هیئت علمی گروه بیماری شناسی گیاهی دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران

4 استاد بازنشسته دانشگاه صنعتی اصفهان، دکتری و فوق­ تخصص گیاهپزشکی، از دانشگاه کالیفرنیا

10.22092/jaep.2019.125722.1278

چکیده

ویروس کوتولگی سبزرد نخود (Chickpea chlorotic dwarf virus, CpCDV) از جنس Mastrevirus، تیره Geminiviridae، از مهمترین عوامل ویروسی همراه با عارضه زردی نخود می‌باشد. در سال­ های 1395-1394 تعداد 248 نمونه نخود و عدس با نشانه زردی از پنج استان‌‌ لــرستان، کــرمانشاه، زنجــان، آذربایجان شرقی و غربی انتخاب و از طریق آزمون TBIA و PCR  بررسی شدند. استخراج دی­ان­ای از نمونه­ های آلوده انجام و از طریق روش دایره غلطان (RCA) تغلیظ و پس از هضم آنزیمی محصول (RCA) با استفاده از آنزیم برشی Xho I ، قطعات با اندازه حدود 6/2 کیلو جفت باز روی ژل آگاروز مشاهده شد. با استفاده از جفت آغازگر اختصاصی و انجام PCR، قطعه 960 جفت بازی تکثیر و پس از همسانه‌سازی، تعیین ترادف گردید. نتایج آزمون TBIA و PCR نشان‌دهنده پراکنش ویروس CpCDV در هر پنج استان بود. بیشترین میزان آلودگی در میزبان نخود مشاهده شد. مقایسه ترادف قطعه ژنومی جدایه­ های ایرانی CpCDV نشانگر بیشترین یکسانی با سویه A، D و F از کشورهای هند، پاکستان و یمن بود. آنالیز تبارزائی نشانگر هم گروهی جدایه­های ایرانی با سویه‌های D (جدایه­ های سودان، مراکش، هند و پاکستان (گروه III تبارزائی)، سویه F (جدایه­ های سودان، عمان، سوریه، یمن و پاکستان (گروه IV تبارزائی) و سویه A  (جدایه‌های تونس، مصر، سوریه و تــرکیه (گروه V تبارزائی ) بود.

کلیدواژه‌ها


عنوان مقاله [English]

Molecular characterization and geographical distribution of Chickpea chlorotic dwarf virus in Iran

نویسندگان [English]

  • Kaveh Bananej 1
  • Yalda Sokhansanj 2
  • Farshad Rakhshandehroo 3
  • Ali Ahoonmanesh 4
2 Department of Plant Protection, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 Faculty member
4 Foulad Institute Technology (FIT), Foulad-shahr, Isfahan-Iran.
چکیده [English]

Chickpea chlorotic dwarf virus (CpCDV, genus Mastrevirus, family Geminiviridae) is one of the most important viral agents which associated with yellowing symptom in the chickpea fields. During the surveys, 2015-2016, 248 chickpea and lentil plant samples showing yellowing symptom were collected from five provinces: Lorestan, Kermanshah, Zanjan, East and West Azarbaijan, in Iran. The samples were tested to CpCDV infection, using TBIA and positive samples in TBIA were tested by PCR. Total DNA of positive samples were extracted and enriched using rolling-circle amplification (RCA). RCA products were restricted using Xho I enzyme and yielding products of ~2.5-6 kb. These fragments were used for polymerase chain reaction (PCR), using specific primers for RepA protein of CpCDV. PCR amplicons with an expected size of ~960 bp were amplified, cloned and sequenced.TBIA and PCR results showed the presence of CpCDV infection in all surveyed provinces with the highest infection in chickpea plants. Nucleotide sequence comparisons showed that Iranian isolates shared the highest identity to strain A, D and F from India, Pakistan, and Yemen. Phylogenetic anlyses showed that Iranian CpCDV strains were grouped with Sudan, Morrocco, India, and Pakistan (strain D, group III), Sudan, Oman, Yemen, and Pakistan (starin F, GroupIV), and Tunesia, Egypt, Syria, and Turkey (strain A, group V).
 

کلیدواژه‌ها [English]

  • Chickpea chlorotic dwarf virus
  • chickpea yellowing symptom
  • geographical distribution

 

AKHTAR, K. P., AHMAD, M., SHAH, T. M., ATTA, B. M. 2011. Transmission of Chickpea chlorotic dwarf virus in chickpea by the leafhopper Orosius albicinctus (Distant) in Pakistan. Plant Protect. Science, No. 47: 1–4.

AKHTAR, S., KHAN, A. J., BRIDDON, R. W. 2013. A distinct strain of Chickpea chlorotic dwarf virus infecting  pepper in Oman. Plant Disease, No. 98: 286-286.

 

ANONYMOUS, 2017. Statistical annual report of Iranian agricultural crops. Ministry of Agriculture Jihad, NO. 40(1): 53.

FAHMY, I. F., TAHA, O., EL-ASHRY, A. N. 2015. First genome analysis and molecular characterization of

Chickpea chlorotic dwarf virus Egyptian isolate infecting squash. Virus Diseases, No. 26: 33–41.

FAOSTAT, 2014. Prunus production: National Agricultural statistics Service, USDA.

FAO, 2014. FAOSTAT Database results from FAO website. Food and Agriculture Organization of the United Nations.

FARZADFAR, S., POURRAHIM, R., GOLNARAGHI, A. R., AHOONMANESH, A. 2008. PCR detection and partial molecular characterization of Chickpea chlorotic dwarf virus in naturally infected sugar beet plants in Iran. Jouranl of Plant Pathology, No. 90: 247-251.

FIALLO-OLIVE, E., MOHAMMED, I. U., TURAKI, A. A., MUHAMMAD, A., NAVAS-CASTILLO, J. A. 2017.Novel Strain of the mastrevirus Chickpea chlorotic dwarf virus infecting papaya in Nigeria. Plant Disease, No. 101: 1684.

HAMZA, M., TAHIR, M. N., MUSTAFA, R., KAMAL, H., KHAN, M. Z., MANSOOR, S., BRODDON, R.W., AMIN, I. 2018. Identification of a dicot infecting mastrevirus along with alpha-and betasatellite associated with leaf curl disease of spinach (Spinacia oleracea) in Pakistan. Virus Research, No. 256: 174–182.

HORN, N. M., REDDY, S. V., REDDY, D. V. R. 1994. Virus-vector relationships of Chickpea chlorotic dwarf geminivirus and the leafhopper Orosius orientalis (Hemiptera: Cicadellidae). Ann. Appl. Biol., No. 124:  441–450.

HORN, N. M., REDDY, S. V., REDDY, D. V. R. 1995. Assessment of yield losses caused by Chickpea chlorotic dwarf geminivires in chickpea (Cicer arientinum) in India. European Journal of Plant Pathology, No. 101: 221-224.

HORN, N. M., REDDY, S. V., ROBERTS, I. M., REDDY, D. V. R. 1993. Chickpea chlorotic dwarf virus, a new leafhopper-transmitted geminivirus of chickpea in India. Ann. Appl. Biol., No. 122: 467-479.

HORN, N. M., REDDY, S. V., van den Huevel, J. F. J. M., and REDDY, D. V. R.  1996. Survey of chickpea (Cicer arietinum L.) for chickpea stunt disease and associated viruses in India and Pakistan. Plant Disease, NO.80: 286-290.

KANAKALA, S., KURIA, P. 2019. Chickpea chlorotic dwarf virus: An Emerging Monopartite Dicot Infecting Mastrevirus. Viruses, No. 11(1): 5-20.

KIMURA, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, No. 16:111-120.

KRABERGER, S., HARKINS, G. W., KUMARI, S.G., THOMAS, J. E., SCHWINGHAMER, M. W., SHARMAN, M., COLLINGS, D. A., BRIDDON, R.W., MARTIN, D. P., VARSANI, A. 2013. Evidence that dicot-infecting mastreviruses are particularly prone to inter-species recombination and have likely been circulating in Australia for longer than in Africa and the Middle East. Virology, NO.444: 282-291.

KRABERGER, S., KUMARI, S. G., HAMED, A. A., GRONENBORN, B., THOMAS, J. E., SHARMAN, M., HARKINS, G. W., MUHIRE, B. M., MARTIN, D. P., VARSANI, A. 2015. Molecular diversity of Chickpea chlorotic dwarf virus in Sudan: High rates of intra-species recombination – a driving force in the emergence of new strains. Infection, Genetics and Evolution, No. 29: 203-215.

KUMARI, S. G., MAKKOUK, K. M., ATTAR, N., GHULAM, W. 2004. First report of Chickpea chlorotic dwarf virus infecting spring chickpea in Syria. Plant Disease, No. 88: 424.

MAKKOUK, K. M., COMEAUE, A. 1994. Evaluation of various methods for the detection of Barley yellow dwarf luteovirus by the tissueblot immunoassay and its use for BYDV detection in cereals inoculated at different growth stages. Europian Journal of  Plant Pathololigy , No. 100: 71-80.

MAKKOUK, K. M., FAZLALI, Y., KUMARI, S. G., and FARZADFAR, S. 2002. First record of Beet western yellows virus, Chickpea chlorotic dwarf virus, Faba bean necrotic yellows virus and Soybean dwarf virus infecting chickpea and lentil crops in Iran. Plant Pathology, No. 51: 387-387.

MAKKOUK, K. M., KUMARI, S. G., SHAHRAEEN, N., FAZLALI, Y., FARZADFAR, S., GHOTBI, T., MANSOURI, A. R. 2002. Identification and seasonal variation of viral diseases of chickpea and lentil in Iran. Journal of Plant Diseases and Protection, No. 110(2): 157–169.

MUMTAZ, H., KUMARI, S. G., MANSOOR, S., MARTIN, D. P., BRIDDON, R.W. 2011. Analysis of the sequence of a dicot-infecting mastrevirus (Family Geminiviridae) originating from Syria. Viris Genes, No. 42: 422-428.

NAHID, N., AMIN, I., MANSOOR, S., RYBICKI, E. P., VAN DER WALT, E., BRIDDON, R. W. 2008. Two dicot-infecting mastreviruses (family Geminiviridae) occur in Pakistan. Archive of Virology, No. 153: 1441-1451.

SAITOU, N., NEI, M., 1987. The neighbour-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. No. 4: 406-425.

TAMURA, K., STECHER, G., PETERSON, D., FILIPSKI, A., KUMAR, S., 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution. No. 30(12): 2725-2729.

OUATTARA, A., TIENDREBEOGO, F., LEFEUVRE, P., HOAREAU, M., CLAVERIE, S., TRAORE, E. V., BARRO, N., TRAORE, O., VARSANI, A., LETT, J. M. 2017. New strains of Chickpea chlorotic dwarf virus discovered on diseased papaya and tomato plants in Burkina Faso. Archive of Virology, No. 162: 1791–1794.

OWOR, B. E., SHEPHERD, D. N., TAYLOR, N. J., EDEMA, R., MONJANE, A. L., THOMSON, J. A., MARTIN, D. P., VARSANI, A. 2007. Successful application of FTA® Classic Card technology and use of bacteriophage φ29 DNA polymerase for large-scale field sampling and cloning of complete maize streak virus genomes. Journal of Virological Methods, No. 140:  100–105.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

AKHTAR, K. P., AHMAD, M., SHAH, T. M., ATTA, B. M. 2011. Transmission of Chickpea chlorotic dwarf virus in chickpea by the leafhopper Orosius albicinctus (Distant) in Pakistan. Plant Protect. Science, No. 47: 1–4.

AKHTAR, S., KHAN, A. J., BRIDDON, R. W. 2013. A distinct strain of Chickpea chlorotic dwarf virus infecting  pepper in Oman. Plant Disease, No. 98: 286-286.

ANONYMOUS, 2017. Statistical annual report of Iranian agricultural crops. Ministry of Agriculture Jihad, NO. 40(1): 53.

FAHMY, I. F., TAHA, O., EL-ASHRY, A. N. 2015. First genome analysis and molecular characterization of

Chickpea chlorotic dwarf virus Egyptian isolate infecting squash. Virus Diseases, No. 26: 33–41.

FAOSTAT, 2014. Prunus production: National Agricultural statistics Service, USDA.

FAO, 2014. FAOSTAT Database results from FAO website. Food and Agriculture Organization of the United Nations.

FARZADFAR, S., POURRAHIM, R., GOLNARAGHI, A. R., AHOONMANESH, A. 2008. PCR detection and partial molecular characterization of Chickpea chlorotic dwarf virus in naturally infected sugar beet plants in Iran. Jouranl of Plant Pathology, No. 90: 247-251.

FIALLO-OLIVE, E., MOHAMMED, I. U., TURAKI, A. A., MUHAMMAD, A., NAVAS-CASTILLO, J. A. 2017.Novel Strain of the mastrevirus Chickpea chlorotic dwarf virus infecting papaya in Nigeria. Plant Disease, No. 101: 1684.

HAMZA, M., TAHIR, M. N., MUSTAFA, R., KAMAL, H., KHAN, M. Z., MANSOOR, S., BRODDON, R.W., AMIN, I. 2018. Identification of a dicot infecting mastrevirus along with alpha-and betasatellite associated with leaf curl disease of spinach (Spinacia oleracea) in Pakistan. Virus Research, No. 256: 174–182.

HORN, N. M., REDDY, S. V., REDDY, D. V. R. 1994. Virus-vector relationships of Chickpea chlorotic dwarf geminivirus and the leafhopper Orosius orientalis (Hemiptera: Cicadellidae). Ann. Appl. Biol., No. 124:  441–450.

HORN, N. M., REDDY, S. V., REDDY, D. V. R. 1995. Assessment of yield losses caused by Chickpea chlorotic dwarf geminivires in chickpea (Cicer arientinum) in India. European Journal of Plant Pathology, No. 101: 221-224.

HORN, N. M., REDDY, S. V., ROBERTS, I. M., REDDY, D. V. R. 1993. Chickpea chlorotic dwarf virus, a new leafhopper-transmitted geminivirus of chickpea in India. Ann. Appl. Biol., No. 122: 467-479.

HORN, N. M., REDDY, S. V., van den Huevel, J. F. J. M., and REDDY, D. V. R.  1996. Survey of chickpea (Cicer arietinum L.) for chickpea stunt disease and associated viruses in India and Pakistan. Plant Disease, NO.80: 286-290.

KANAKALA, S., KURIA, P. 2019. Chickpea chlorotic dwarf virus: An Emerging Monopartite Dicot Infecting Mastrevirus. Viruses, No. 11(1): 5-20.

KIMURA, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, No. 16:111-120.

KRABERGER, S., HARKINS, G. W., KUMARI, S.G., THOMAS, J. E., SCHWINGHAMER, M. W., SHARMAN, M., COLLINGS, D. A., BRIDDON, R.W., MARTIN, D. P., VARSANI, A. 2013. Evidence that dicot-infecting mastreviruses are particularly prone to inter-species recombination and have likely been circulating in Australia for longer than in Africa and the Middle East. Virology, NO.444: 282-291.

KRABERGER, S., KUMARI, S. G., HAMED, A. A., GRONENBORN, B., THOMAS, J. E., SHARMAN, M., HARKINS, G. W., MUHIRE, B. M., MARTIN, D. P., VARSANI, A. 2015. Molecular diversity of Chickpea chlorotic dwarf virus in Sudan: High rates of intra-species recombination – a driving force in the emergence of new strains. Infection, Genetics and Evolution, No. 29: 203-215.

KUMARI, S. G., MAKKOUK, K. M., ATTAR, N., GHULAM, W. 2004. First report of Chickpea chlorotic dwarf virus infecting spring chickpea in Syria. Plant Disease, No. 88: 424.

MAKKOUK, K. M., COMEAUE, A. 1994. Evaluation of various methods for the detection of Barley yellow dwarf luteovirus by the tissueblot immunoassay and its use for BYDV detection in cereals inoculated at different growth stages. Europian Journal of  Plant Pathololigy , No. 100: 71-80.

MAKKOUK, K. M., FAZLALI, Y., KUMARI, S. G., and FARZADFAR, S. 2002. First record of Beet western yellows virus, Chickpea chlorotic dwarf virus, Faba bean necrotic yellows virus and Soybean dwarf virus infecting chickpea and lentil crops in Iran. Plant Pathology, No. 51: 387-387.

MAKKOUK, K. M., KUMARI, S. G., SHAHRAEEN, N., FAZLALI, Y., FARZADFAR, S., GHOTBI, T., MANSOURI, A. R. 2002. Identification and seasonal variation of viral diseases of chickpea and lentil in Iran. Journal of Plant Diseases and Protection, No. 110(2): 157–169.

MUMTAZ, H., KUMARI, S. G., MANSOOR, S., MARTIN, D. P., BRIDDON, R.W. 2011. Analysis of the sequence of a dicot-infecting mastrevirus (Family Geminiviridae) originating from Syria. Viris Genes, No. 42: 422-428.

NAHID, N., AMIN, I., MANSOOR, S., RYBICKI, E. P., VAN DER WALT, E., BRIDDON, R. W. 2008. Two dicot-infecting mastreviruses (family Geminiviridae) occur in Pakistan. Archive of Virology, No. 153: 1441-1451.

SAITOU, N., NEI, M., 1987. The neighbour-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. No. 4: 406-425.

TAMURA, K., STECHER, G., PETERSON, D., FILIPSKI, A., KUMAR, S., 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution. No. 30(12): 2725-2729.

OUATTARA, A., TIENDREBEOGO, F., LEFEUVRE, P., HOAREAU, M., CLAVERIE, S., TRAORE, E. V., BARRO, N., TRAORE, O., VARSANI, A., LETT, J. M. 2017. New strains of Chickpea chlorotic dwarf virus discovered on diseased papaya and tomato plants in Burkina Faso. Archive of Virology, No. 162: 1791–1794.

OWOR, B. E., SHEPHERD, D. N., TAYLOR, N. J., EDEMA, R., MONJANE, A. L., THOMSON, J. A., MARTIN, D. P., VARSANI, A. 2007. Successful application of FTA® Classic Card technology and use of bacteriophage φ29 DNA polymerase for large-scale field sampling and cloning of complete maize streak virus genomes. Journal of Virological Methods, No. 140:  100–105.

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