مسمومیت زهر عقرب Hottentotta saulcyi در برابر Sesamia nonagrioides آفت نیشکر

نوع مقاله: حشره شناسی کشاورزی

نویسندگان

1 موسسه تحقیقات واکسن و سرم سازی رازی شعبه جنوب غرب کشور

2 استادیار گروه علوم دامی، دانشکده علوم دامی و صنایع غذایی- دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، اهواز، ایران

3 گروه جانوران سمی و تولید پادزهر، موسسه تحقیقات واکسن و سرم سازی رازی شعبه جنوب غرب کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران

4 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

5 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

10.22092/jaep.2019.122681.1240

چکیده

به منظور ارزیابی سمیت زهر عقرب Hottentotta saulcyi علیه کرم ساقه‌خوار نیشکر (Sesamia nonagrioides)، درصد مرگ و میر لاروها، فعالیت آنزیم‌های سم‌زدایی و تغییرات هیستوپاتولوژی بافت‌های سزمیا مورد بررسی قرار گرفت. سم به لارو‌ها در پنج دوز مختلف 15/0، 35/0، 5/0، 75/0 و 1 میکروگرم (پانزده لارو به ازای هر دوز) تزریق شد و دوزهای که در طول آزمایش سبب مرگ و میر لاروها شده بودند ثبت شدند. این مطالعه نشان داد که زهر H. saulcyi قادر به ایجاد نشانه‌های ظاهری بوده که در نهایت منجر به مرگ لارو‌های مسموم شده گردید. علاوه بر این، این سم به شدت فعالیت
گلوتاتیون S-transferases (GST)  را مهار کرد. داده‌های این آزمایش نشانگر وجود رابطه بین فعالیت آنزیم GST و مرگ و میر لارو می‌باشد، به‌طوری که غیر فعال شدن این آنزیم توسط زهر عقرب ممکن است مسئول افزایش مرگ و میر لارو باشد. در این مطالعه، آسیب‌های غشای روده و لوله‌های مالپیگی با استفاده از روش‌های هیستوپاتولوژیکی بررسی شد. در اثر مسمومیت، تغییرات هیستوپاتولوژیکی واضحی در تمام لاروهای تزریقی با دوز 1 میکرولیتر سم مشاهده شد، از جمله تجزیه و بهم خوردن ساختار سلول‌های غشای روده میانی که با کاهش پیشرونده غشای پریتروفی و ناپدید شدن میکرو ویلی‌ها همراه بود.
 

کلیدواژه‌ها


عنوان مقاله [English]

Toxicity of Hottentotta saulcyi venom against Sesamia nonagrioides on sugarcane plants

نویسندگان [English]

  • fatemeh salabi 1
  • Mahmood Nazari 2
  • Hadiye Jafari 3
  • Ali Shahriari 4
  • Anahita Rezaei 5
1 Razi Vaccine and Serum Research Institute
2 Department of Animal Science, Faculty of Animal science and Food Technology, Khuzestan University of Agricultural Sciences and Natural Resources, Ahvaz, Iran
3 Department of Venomous Animals and Anti-venom Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran
4 Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
5 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده [English]

To evaluate the toxicity of Hottentotta saulcyi venom on Sesamia nonagrioides, we investigated the larval mortality, detoxification enzyme activity and histopathological alterations of the S. nonagrioides organs. Venoms were injected into the larvae at five doses (0.15µg, 0.35µg, 0.5µg, 0.75µg and 1µg; fifteen larvae per each dose) and the doses causing death were recorded during the experiment. This study demonstrated that H. saulcyi venom was able to cause external symptoms that eventually led to death of the intoxicated larvae. Moreover, this venom strongly inhibited glutathione S-transferases (GST) activity. Our data highlighted the relationship between the activity of GST enzyme and larval mortalities, so that the inactivation of this enzyme by scorpion venom may be responsible for the increasing the larval mortality. In the present study, the damage of midgut epithelium and malpighian tubules has been directly visualized and documented using histopathology methods. Clear histopathological alterations of intoxication were observed for all larvae injected with 1µg doses of venom, including a distinctive lysing and disorganizing of midgut epithelial cells that coupled with a progressive loss of the peritrophic membrane and the disappearance of microvilli.

کلیدواژه‌ها [English]

  • Keywords: Glutathione S-transferases
  • Hottentotta saulcyi
  • malpighian tubules
  • Midgut
  • Sesamia nonagrioides

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